nucleofector solution v (Amaxa)

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Amaxa nucleofector solution v
Nucleofector Solution V, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nucleofector+solution/nucleofector+solution+v/us12616759-1694-9-12
Average 86 stars, based on 1 article reviews

nucleofector solution v - by Bioz Stars, 2026-10

86/100 stars

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shRNA:

Article Title: The HRAS-binding C2 domain of PLCη2 suppresses tumor-like synoviocytes and experimental arthritis in rheumatoid arthritis.
Article Snippet: The DNA and shRNA vectors were transfected separately into cells using the Human Dermal Fibroblast Nucleofector Kit (Lonza) according to the manufacturer’s protocol. .. In brief, 5 × 105 cells were resuspended in 100 μl of nucleofector solution containing DNA or shRNA plasmids and electroporated with the U-23 program on an Amaxa Nucleofector II device. ..

Transfection:

Article Title: An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission.
Article Snippet: Schizonts were obtained by culturing 500 μl blood containing >1% parasitaemia in schizont medium (RPMI-1640 (Gibco, 52400-025) supplemented with 20% fetal calf serum (FCS) (Gibco, 26140-079)) and 1 μg ml−1 gentamycin (PAA) for ~20 h at 37 °C, and were then purified over a 55% Nycodenz (Axis-Shield Diagnostics) gradient. .. DNA and schizonts were mixed with 100 μl Nucleofector solution (either from the parasite transfection kit or from the human T cell Nucleofector kit), electroporated using the Amaxa Nucleofector II device (Lonza) and immediately injected intravenously (i.v.) into a mouse. .. Transgenic parasites were selected by administering pyrimethamine (7 μg ml−1, Sigma-Aldrich) to the drinking water 1 day after transfection.

Injection:

Article Title: A genetically encoded marker for imaging membranes of Plasmodium
Article Snippet: Schizonts were obtained by culturing 500 μl blood containing > 1% parasitemia in schizont medium (RPMI-1640, gibco) supplemented with 20% FCS (gibco) and 1 μg/ml gentamycin (PAA) for about 20 h at 37 °C and were purified over a 55% Nycodenz (Axis-shield diagnostics) gradient. .. DNA and schizonts were mixed with 100 μl Nucleofector solution from the human T cell Nucleofector Kit, electroporated using the Amaxa Nucleofector II device (Lonza), and immediately injected intravenously into a mouse. .. Transgenic parasites were selected by administering pyrimethamine (7 μg/ml, Sigma-Aldrich) to the drinking water one day after transfection.

other:

Article Title: HDAC6-Mediated FoxO1 Acetylation And Phosphorylation Control Periodontal Inflammatory Responses
Article Snippet: Briefly, 4 × 10 6 MEFs were re-suspended in 100 μl of Nucleofector solution (MEF Nucleofector kit; Amaxa) together with 2μg of a green fluorescent protein (GFP)-coding plasmid (pCMV-GFP) and 2μg of siRNA duplexes for each target.

Plasmid Preparation:

Article Title: AGS3-based optogenetic GDI induces GPCR-independent Gβγ signalling and macrophage migration.
Article Snippet: .. Nucleofector solution (82 μl), supplement solution (18 μl) from Amaxa Cell Line Nucleofector Kit V and appropriate volumes of plasmid DNA for specific DNA combinations were mixed. ..

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